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Molecular identification and genetic diversity of apple snail Pila globosa by RAPD assay


A.K.M.Al-Amin Leamon[1],Shad Ebna Rahaman[2], Shamita Mahzabin[1], Abdullah Maruf Rahman Shovo[2] ,Md. Faruque Miah [1]
Page No. 207-217


Abstract

This study was carried out for molecular identification and genetic diversity of freshwater snail Pila
globosa in Bangladesh. Traditional taxonomy of different species of the genus Pila has always been
obscured and unreliable due to their morphological ambiguity. A rapid and cost effective molecular
marker, mitochondrial COI gene was used to establish molecular standards for identification while
similar bands were seen in all individuals of Pila globosa at the level of 700 bp of lengths. In
addition, genetic diversity of freshwater snail Pila globosa was measured on the basis of PCRRAPD
assay. Three arbitrary primers were screened and 12 polymorphic loci were found in 7
individuals of this experimental species. The percentage of average values of polymorphic loci and
polymorphism information content found 21.23 and 0.875 respectively. Highest and lowest Nei
genetic similarity was found 0.500 and 0.105 respectively whereas genetic distance was recorded
between 0.667 and 0.9444. Furthermore, the highest and lowest linkage distance was observed 17.0
and 9.0 respectively. A distant relationship among individuals was found by studying phylogenetic
dendrogram. Though higher genetic diversity was observed in Pila globosa, however, the
population of this species in the nature of Bangladesh is not confirm with good genetic status while
only seven individuals were investigated.
Key words: Molecular identification, genetic diversity, Pila globosa, PCR-RAPD


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