Nanotech Combination Chemoprevention Study Against Balb/c Mice Implanted
with Sarcoma 180 Cancer Cells

Sharad Medhe, Prachi Bansal and ManMohan Srivastava*
Page No. 1-12

Cancer is one of the most dreaded diseases in 21st century. Nanoparticle based therapeutics exhibit
more benefits when compared to its active phytoconstituent alone. It also widely used as an efficient
chemotherapeutic agent for the treatment of cancer and related diseases. The present work has
designed to test in vivo anticancer activity of gold nanoparticle embeded 3,6-dihydroxyflavone
(GNDHF); lutein (LUT) and selenium methyl selenocysteine (MSC) in single and combination
against sarcoma 180 cancer cells induced in female inbred Balb/c mice strain. In vivo anticancer
activity was evaluated by antigenotoxic (Chromosomal aberrations test, Micronucleus test) and
Physical observation ((Body weight, tumor volume and tumor delay time)) of Balb/c mice strain.
The antioxidant assays were determined by reduced glutathione (GSH) and Lipid Peroxidation
(LPO) assay. Among the various treatment considered for the study, combination of (GNDHF: LUT:
MSC) 5mg/Kg body weight dose exhibited optimum antioxidant activity (0.11±0.54 μM) GSH per
mg protein while (0.187±0.74 nM) MDA per mg wet weight and rendered significant protection
against oxidative stress induced by sarcoma 180 cancer cells in liver tissues. Combination
(GNDHF: LUT: MSC) significantly suppresses the tumor growth in terms of body weight: 28.12
gm±1.04; tumor volume; 70.12 mm3 (42 % inhibition) at 5mg/Kg body weight dose. The study
highlights that the combination of (GNDHF: LUT: MSC) is more promising cytotoxic bioagent
against sarcoma 180 cancer cells induced in female Balb/c mice compared to the single compound
and has enough potential for clinical applications.
Key words: Nanoparticle; Combination study; in vivo; Oxidative stress.